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CytoArchitec lab

  • English
  • Japanese
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    • Japanese
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    CytoArchitec lab

    • English
    • Japanese
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      • English
      • Japanese
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      • Kyushu University, Faculty of Design

        Cytoskeletal Architecture

        and

        Bio-Self-Assembly lab

      • Design inspired by self-organization in biosystem

        Self-organization is a process in which local interaction between components of a system spontaneously produce ordered structures and fascinating patterns which exhibit emergent functions in the absence of a professional designer. This is a common assembly process in a biosystem and can be co-opted by scientists and designers based on different construction principles and logics of design. Since self-organization grants several advantages to the system including robustness, flexibility and adaptability to the surrounding environment, innovations based on this self-organization may provide a unique avenue for designers and inventors.

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        Biomaterials: Cytoskeletons & Motor proteins

        Biophysics, Micro/Nanotechnology

        The study of self-organization in a biosystem, a system comprised of skeletons in cells so-called as cytoskeleton (microtubules and actin) and its related motor proteins, provides one with unique tools and design principles not seen in other systems. The biosystem is one of the best candidates for innovation since they autonomously form various structures, and determine the morphology of the cell wherein they lie. This allows a designer with the ability to innovate with these self-assembling structures, potentially bringing this microscopic design into the macroscopic world. We are studying it using the reconstituted system of cytoskeleton combining with micro/nanotechnologies.

        Artificial materials

        Interior, Architecture design

        To explore potential applications of self-organization, our other challenge is to develop artificial systems organized through self-organization at human scales inspired by cytoskeletons.

      • Basic Equipments & technologies

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        Nikon Ti2-E,
        Epi-fluorescence microscope

        Fluorescence microscopy imaging

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        Optline TIRF ReLIEF

        A total internal reflection fluorescence microscope to observe thin region of a specimen, usually less than 200 nanometers

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        SPM/AFM SPA300HV 

        Atomic force microscope to visualize surface molecular structure of materials at nm resolution

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        Formlabs Form3,

        3D printer

        3D print at high resolution
        (0.025 mm) using Low Force Stereolithography (LFS)

      • Cover art & BIOART Gallery 

        Journal cover of Nano Letters, 2024, 24, 35, 10790–10795
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      • Toilet paper indicates actin filaments polymerizing at cell front. Smou wrestler is myosin.
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        ZIgzag pattern of gliding microtubules formed under cyclic stretching
        Active nematic of taxol stabilized microtubules polymerized by GTP
        RIng-shaped microtubule assemblies
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        Gliding microtubules over kinesin coated surface observed by IRM
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        Steampunk Molecular Chaperone, prototype
        Steampunk Molecular Chaperone, prototype
        Motility assay of 3D printed Steampunk Dynein along tubulin protofilament
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        3D printing by using Silk Worm
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        ACT48 drawn by Midjourney AI
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      • Selected Papers

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        Surface Passivation of Norland Optical Adhesive Improves the Guiding Efficiency of Gliding Microtubules in Microfluidic Devices

        Inoue, D. 

        Nano Lett. 2024, 24, 35, 10790–10795

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        In Vitro Synthesis and Design of Kinesin Biomolecular Motors by Cell-Free Protein Synthesis

        Inoue, D.; Ohashi, K.; Takasuka, E. T.; Kakugo, A.

        ACS Synthetic Biology 2023, 12, 6, 1624-1631

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        Design X Bioinformatics: a community-driven initiative to connect bioinformatics and design

        Sommer, B.*; Inoue, D.; Baaden, M.

        Journal of Integrative Bioinformatics,2022,19

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        Monopolar flocking of microtubules
        in collective motion

        Afroze, F.†; Inoue, D.†; Farhana, T. I.; Hiraiwa, T.; Akiyama, R.; Kabir A. M. R.; Sada, K.; Kakugo, A.*
        (†Contribution equal)

        Biochemical and Biophysical Research Communications 2021, 563,73-78

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        Self-repair protects microtubules from destruction by molecular motors

        Triclin S.†, Inoue D.†​, Gaillard J., Htet Z. M., DeSantis M. E., Portran D., Derivery E., Aumeier C., Schaedel L., John K., Leterrier C., Reck-Peterson S. L., Blanchoin L.* & Théry M.* (†Contribution equal)

        Nature Materials 2021, 20,883-891

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        Mechanical stimulation‐induced orientation of gliding microtubules in confined microwells

        Inoue, D.; Kabir, A. M. R.; Tokuraku, K.; Sada, K.; Kakugo, A.

        Advanced Materials Interfaces 2020, 7, 1902013

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        Adaptation of patterns of motile filaments under dynamic boundary conditions

        Inoue, D.; Gutmann, G.; Nitta, T.; Kabir, A. M. R.; Konagaya, A.; Tokuraku, K.; Sada, K.; Hess, H.; Kakugo, A.

        ACS Nano 2019, 13, 12452-12460

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        Actin filaments regulate microtubule growth at the centrosome

        Inoue, D. †; Obino, D.†; Farina, F.; Gaillard, J.; Guerin, C.; Blanchoin, L.*; Lennon-Duménil, A. M.*; Théry, M.*

        The EMBO Journal 2019, e99630

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        Are microtubules tension sensors?

        Hamant*, O.; Inoue, D.; Bouchez, D.; Dumais, J.; Mjolsness, E.

        Nature Communications 2019, 10, 2369.

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        Construction of artificial cilia from microtubules and kinesins through a designed bottom-up approach

        Sasaki, R.; Kabir, A. M. R.; Inoue, D.; Anan, S.; Kimura, A. P., Konagaya, A.; Sada, K. and Kakugo, A.*

        Nanoscale 2018,10, 6323-6332

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        DNA-assisted swarm control in a biomolecular motor system

        Keya, J.; Suzuki, R.; Kabir, A. M. R.; Inoue, D.; Asanuma, H.; Sada, K.; Hess, H.*; Kuzuya, A.*; Kakugo, A.*

        Nature Communications 2018, 9, 453

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        Depletion force induced collective motion of microtubules driven by kinesin

        Inoue, D.; Mahemuti, B.; Kabir, A. M. R.; Farhana, T. I.; Tokuraku, K.; Sada, K.; Konagaya, A.; Kakugo, A.*

        Nanoscale 2015, 7, 18054-18061

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        High-resolution imaging of a single gliding protofilament of tubulins by HS-AFM

        Keya, J. J.†; Inoue, D.†; Suzuki, Y.; Kozai, T.; Ishikuro, D.; Kodera, N.; Uchihashi, T.; Kabir, A. M. R.; Endo, M.; Sada, K.; Kakugo, A.* (†Contribution equal)

        Scientific Reports 2017, 7, 6166

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        Sensing surface mechanical deformation using active probes driven by motor proteins

        Inoue, D.; Nitta, T.; Kabir, A. M. R.; Sada, K.; Gong, J.P.; Konagaya, A.; Kakugo, A.*

        Nature Communications 2016, 7, 12557

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        Other publications are available here

      • Free Materials for BioArt

        Anyone can use those materials for free. But please don't redistribute these items without modification.

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        Brush for Clip Studio (Coming soon)
      • Member

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        Daisuke Inoue, (Lab PI)

        Assistant Professor

        CV

      • 2024 member

        2024年メンバー
        April 22, 2024
        学部4年生 ・佐野開音 ・末松春樹  
      • Contact

        Kyushu University, Ohashi campus
        Room 605, Building 3, Shiobaru 4-9-1, Minami-Ku, Fukuoka, 815-8540, Japan
        (+81) 92-553-4431
        (+81) 92-553-4431
        dinoue1@design.kyushu-u.ac.jp
      • Related labs

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